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Diagnostic Bacteriology

Overview of attention for book
Cover of 'Diagnostic Bacteriology'

Table of Contents

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    Book Overview
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    Chapter 1 Whole-Genome Enrichment Using RNA Probes and Sequencing of Chlamydia trachomatis Directly from Clinical Samples
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    Chapter 2 Characterization of Sinus Microbiota by 16S Sequencing from Swabs
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    Chapter 3 Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR)
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    Chapter 4 Detection of Helicobacter pylori DNA in Formalin-Fixed Paraffin-Embedded Gastric Biopsies Using Laser Microdissection and qPCR
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    Chapter 5 Mycobacterial Load Assay
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    Chapter 6 Defining Diagnostic Biomarkers Using Shotgun Proteomics and MALDI-TOF Mass Spectrometry
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    Chapter 7 Detection and Typing of “Candidatus Phytoplasma ” spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres
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    Chapter 8 Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization
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    Chapter 9 Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections
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    Chapter 10 Detection and Differentiation of Lyme Spirochetes and Other Tick-Borne Pathogens from Blood Using Real-Time PCR with Molecular Beacons
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    Chapter 11 Methods for Real-Time PCR-Based Diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus Infections in an Opened Molecular Diagnostic Platform
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    Chapter 12 Real-Time PCR to Identify Staphylococci and Assay for Virulence from Blood
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    Chapter 13 Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis
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    Chapter 14 A Closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
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    Chapter 15 Highly Specific Ligation-dependent Microarray Detection of Single Nucleotide Polymorphisms
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    Chapter 16 Multilocus Sequence Typing (MLST) for Cronobacter spp.
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    Chapter 17 Diagnostic Bacteriology: Raman Spectroscopy
Attention for Chapter 14: A Closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
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Chapter title
A Closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
Chapter number 14
Book title
Diagnostic Bacteriology
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7037-7_14
Pubmed ID
28600772
Book ISBNs
978-1-4939-7035-3, 978-1-4939-7037-7
Authors

Marcos D. Trangoni, Andrea K. Gioffré, Silvio L. Cravero, Trangoni, Marcos D., Gioffré, Andrea K., Cravero, Silvio L.

Editors

Kimberly A. Bishop-Lilly

Abstract

LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, with the blueprint that it can be implemented in laboratories of low technological complexity. Despite the conceptual complexity underlying the mechanistic basis for the nucleic acid amplification, the technique is simple to use and the amplification and detection can be carried out in just one step. In this chapter, we present a protocol based on LAMP for the rapid identification of isolates of Brucella spp. and Mycobacterium avium subsp. paratuberculosis, two major bacterial pathogens in veterinary medicine.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 18%
Student > Master 2 12%
Student > Doctoral Student 1 6%
Lecturer 1 6%
Other 1 6%
Other 1 6%
Unknown 8 47%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 2 12%
Medicine and Dentistry 2 12%
Immunology and Microbiology 2 12%
Nursing and Health Professions 1 6%
Unspecified 1 6%
Other 1 6%
Unknown 8 47%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2017.
All research outputs
#18,554,389
of 22,979,862 outputs
Outputs from Methods in molecular biology
#7,946
of 13,148 outputs
Outputs of similar age
#241,744
of 317,056 outputs
Outputs of similar age from Methods in molecular biology
#184
of 303 outputs
Altmetric has tracked 22,979,862 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,148 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 317,056 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 303 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.

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