Chapter title |
Detection and Typing of “Candidatus Phytoplasma ” spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres
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Chapter number | 7 |
Book title |
Diagnostic Bacteriology
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Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7037-7_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7035-3, 978-1-4939-7037-7
|
Authors |
Edel Pérez-López, Christine Hammond, Chrystel Olivier, Tim J. Dumonceaux, Pérez-López, Edel, Hammond, Christine, Olivier, Chrystel, Dumonceaux, Tim J. |
Editors |
Kimberly A. Bishop-Lilly |
Abstract |
The use of oligonucleotide-coupled fluorescent microspheres is a rapid, sequencing-independent, and reliable way to diagnose bacterial diseases. Previously described applications of oligonucleotide-coupled fluorescent microspheres for the detection and identification of bacteria in human clinical samples have been successfully adapted to detect and differentiate "Ca. Phytoplasma" species using as a target the chaperonin 60-encoding gene. In this chapter, we describe in detail the design and validation of oligonucleotide capture probes, and their application in the assay aiming to differentiate phytoplasma strains infecting Brassica napus and Camelina sativa plants grown in the same geographic location at the same time. |
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Members of the public | 3 | 100% |
Mendeley readers
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Unknown | 8 | 100% |
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Researcher | 1 | 13% |
Other | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 5 | 63% |
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Agricultural and Biological Sciences | 3 | 38% |
Unknown | 5 | 63% |