| Chapter title |
Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus
|
|---|---|
| Chapter number | 3 |
| Book title |
Reverse Genetics of RNA Viruses
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6964-7_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6962-3, 978-1-4939-6964-7
|
| Authors |
Mingyuan Han, Hanzhong Ke, Yijun Du, Qingzhan Zhang, Dongwan Yoo, Han, Mingyuan, Ke, Hanzhong, Du, Yijun, Zhang, Qingzhan, Yoo, Dongwan |
| Editors |
Daniel R. Perez |
| Abstract |
Porcine reproductive and respiratory syndrome (PRRS) is a reemerging swine disease, and has become economically the most significant disease in pork production worldwide. The causative agent is PRRS virus (PRRSV), which is a member virus of the family Arteriviridae. The PRRSV genome is a single-stranded positive-sense RNA and is infectious. Two strategies in the PRRSV reverse genetics system have been employed for reconstitution of progeny virus: RNA transfection and DNA transfection. The PRRSV reverse genetics has broadly been used for studies including protein structure-function relationship, foreign gene expression, vaccine development, virulence determinants, and viral pathogenesis. Herein, we describe the modification of the pFL12 "RNA launch" reverse genetic system to the CMV promoter-driven pXJ41-FL13 "DNA launch" system. The generation of progeny PRRSV using pXJ41-FL13 is further elucidated. |
X Demographics
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| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 27% |
| Professor | 2 | 18% |
| Student > Bachelor | 2 | 18% |
| Other | 1 | 9% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 3 | 27% |
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Agricultural and Biological Sciences | 1 | 9% |
| Immunology and Microbiology | 1 | 9% |
| Unknown | 4 | 36% |