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Reverse Genetics of RNA Viruses

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Attention for Chapter 8: BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV)
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Chapter title
BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV)
Chapter number 8
Book title
Reverse Genetics of RNA Viruses
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6964-7_8
Pubmed ID
Book ISBNs
978-1-4939-6962-3, 978-1-4939-6964-7
Authors

Christopher C. Stobart, Anne L. Hotard, Jia Meng, Martin L. Moore, Stobart, Christopher C., Hotard, Anne L., Meng, Jia, Moore, Martin L.

Editors

Daniel R. Perez

Abstract

Respiratory syncytial virus (RSV) is an enveloped, nonsegmented negative-strand RNA virus, which causes lower respiratory tract infections and is a leading cause of mortality in young infants. There is no available RSV vaccine and currently administered prophylactic antibodies are limited to high-risk populations. Current efforts to develop vaccines include development of live-attenuated RSV candidates. We describe here methods for preparation and recovery of recombinant RSV using an efficient bacterial artificial chromosome (BAC)-based system, expansion and plaque purification of recovered virus, and generation of master and working stocks.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 14%
Student > Ph. D. Student 2 14%
Other 1 7%
Student > Bachelor 1 7%
Researcher 1 7%
Other 1 7%
Unknown 6 43%
Readers by discipline Count As %
Medicine and Dentistry 2 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Agricultural and Biological Sciences 1 7%
Biochemistry, Genetics and Molecular Biology 1 7%
Immunology and Microbiology 1 7%
Other 1 7%
Unknown 7 50%