Chapter title |
BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV)
|
---|---|
Chapter number | 8 |
Book title |
Reverse Genetics of RNA Viruses
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6964-7_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6962-3, 978-1-4939-6964-7
|
Authors |
Christopher C. Stobart, Anne L. Hotard, Jia Meng, Martin L. Moore, Stobart, Christopher C., Hotard, Anne L., Meng, Jia, Moore, Martin L. |
Editors |
Daniel R. Perez |
Abstract |
Respiratory syncytial virus (RSV) is an enveloped, nonsegmented negative-strand RNA virus, which causes lower respiratory tract infections and is a leading cause of mortality in young infants. There is no available RSV vaccine and currently administered prophylactic antibodies are limited to high-risk populations. Current efforts to develop vaccines include development of live-attenuated RSV candidates. We describe here methods for preparation and recovery of recombinant RSV using an efficient bacterial artificial chromosome (BAC)-based system, expansion and plaque purification of recovered virus, and generation of master and working stocks. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Other | 1 | 7% |
Student > Bachelor | 1 | 7% |
Researcher | 1 | 7% |
Other | 1 | 7% |
Unknown | 6 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Agricultural and Biological Sciences | 1 | 7% |
Biochemistry, Genetics and Molecular Biology | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Other | 1 | 7% |
Unknown | 7 | 50% |