↓ Skip to main content
What is this page? Embed badge

Cell-Free Protein Synthesis

Overview of attention for book
Cover of 'Cell-Free Protein Synthesis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae.
  3. Altmetric Badge
    Chapter 2 Bioinformatics analysis and optimization of cell-free protein synthesis.
  4. Altmetric Badge
    Chapter 3 A cell-free expression screen to identify fusion tags for improved protein expression.
  5. Altmetric Badge
    Chapter 4 One-pot, microscale cell-free enzyme expression and screening.
  6. Altmetric Badge
    Chapter 5 Cell-free translation of biofuel enzymes.
  7. Altmetric Badge
    Chapter 6 Cloning-independent expression and screening of enzymes using cell-free protein synthesis systems.
  8. Altmetric Badge
    Chapter 7 High-level cell-free production of membrane proteins with nanodiscs.
  9. Altmetric Badge
    Chapter 8 Cell-free protein-based enzyme discovery and protein-ligand interaction study.
  10. Altmetric Badge
    Chapter 9 Human cell extract-derived cell-free systems for virus synthesis.
  11. Altmetric Badge
    Chapter 10 Cell-free protein synthesis in microfluidic 96-well plates.
  12. Altmetric Badge
    Chapter 11 Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis.
  13. Altmetric Badge
    Chapter 12 Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis.
  14. Altmetric Badge
    Chapter 13 In Vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate.
  15. Altmetric Badge
    Chapter 14 An optimized yeast cell-free lysate system for in vitro translation of human virus mRNA.
  16. Altmetric Badge
    Chapter 15 In Vitro Translation-Based Protein Kinase Substrate Identification
  17. Altmetric Badge
    Chapter 16 Preparation of Protein Arrays Using Cell-Free Protein Expression
  18. Altmetric Badge
    Chapter 17 Posttranscriptional control of protein synthesis in Drosophila s2 cell-free system.
  19. Altmetric Badge
    Chapter 18 Cell-free membrane protein expression.
  20. Altmetric Badge
    Chapter 19 The PURE System for Protein Production.
  21. Altmetric Badge
    Chapter 20 A cell-free protein synthesis system from insect cells.
  22. Altmetric Badge
    Chapter 21 A cell-free expression platform for production of protein microarrays.
  23. Altmetric Badge
    Chapter 22 ERRATA
Attention for Chapter 19: The PURE System for Protein Production.
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age and source (55th percentile)

Mentioned by

twitter
2 X users

Citations

dimensions_citation
5 Dimensions

Readers on

mendeley
43 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
The PURE System for Protein Production.
Chapter number 19
Book title
Cell-Free Protein Synthesis
Published in
Methods in molecular biology, December 2013
DOI 10.1007/978-1-62703-782-2_19
Pubmed ID
24395424
Book ISBNs
978-1-62703-781-5, 978-1-62703-782-2
Authors

Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, Takuya Ueda, Shimizu, Yoshihiro, Kuruma, Yutetsu, Kanamori, Takashi, Ueda, Takuya

Editors

Kirill Alexandrov, Wayne A. Johnston

Abstract

In the field of molecular biology or biochemistry, preparation and use of purified proteins involved in a certain biological system is crucial for understanding their mechanisms and functions in cells or organisms. The recent progress in a cell-free translation system allows us to prepare proteins in a test tube directly from cDNAs that encode the amino acid sequences. The use of the reconstituted cell-free translation system termed PURE (Protein synthesis Using Recombinant Elements) for these purposes is effective in several applications. Here we describe methods of recombinant protein expression using the PURE system for molecular biological or biochemical studies.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 23%
Student > Ph. D. Student 7 16%
Researcher 4 9%
Student > Master 4 9%
Professor 3 7%
Other 5 12%
Unknown 10 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 19 44%
Agricultural and Biological Sciences 8 19%
Chemistry 3 7%
Unspecified 1 2%
Social Sciences 1 2%
Other 1 2%
Unknown 10 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 January 2014.
All research outputs
#15,289,831
of 22,738,543 outputs
Outputs from Methods in molecular biology
#5,308
of 13,087 outputs
Outputs of similar age
#191,271
of 305,972 outputs
Outputs of similar age from Methods in molecular biology
#195
of 572 outputs
Altmetric has tracked 22,738,543 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,087 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,972 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 572 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.

This page is provided by Altmetric.