Chapter title |
Exploring the Protein Folding Pathway with High-Pressure NMR: Steady-State and Kinetics Studies
|
---|---|
Chapter number | 13 |
Book title |
High Pressure Bioscience
|
Published in |
Sub cellular biochemistry, January 2015
|
DOI | 10.1007/978-94-017-9918-8_13 |
Pubmed ID | |
Book ISBNs |
978-9-40-179917-1, 978-9-40-179918-8
|
Authors |
Julien Roche, Mariano Dellarole, Catherine A. Royer, Christian Roumestand, Roche, Julien, Dellarole, Mariano, Royer, Catherine A., Roumestand, Christian |
Abstract |
Defining the physical-chemical determinants of protein folding and stability, under normal and pathological conditions has constituted a major subfield in biophysical chemistry for over 50 years. Although a great deal of progress has been made in recent years towards this goal, a number of important questions remain. These include characterizing the structural, thermodynamic and dynamic properties of the barriers between conformational states on the protein energy landscape, understanding the sequence dependence of folding cooperativity, defining more clearly the role of solvation in controlling protein stability and dynamics and probing the high energy thermodynamic states in the native state basin and their role in misfolding and aggregation. Fundamental to the elucidation of these questions is a complete thermodynamic parameterization of protein folding determinants. In this chapter, we describe the use of high-pressure coupled to Nuclear Magnetic Resonance (NMR) spectroscopy to reveal unprecedented details on the folding energy landscape of proteins. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 33% |
Professor | 2 | 22% |
Researcher | 2 | 22% |
Student > Master | 1 | 11% |
Student > Doctoral Student | 1 | 11% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 44% |
Chemistry | 2 | 22% |
Physics and Astronomy | 1 | 11% |
Agricultural and Biological Sciences | 1 | 11% |
Unknown | 1 | 11% |