| Chapter title |
Visualizing and Tracking T Cell Motility In Vivo
|
|---|---|
| Chapter number | 3 |
| Book title |
T-Cell Trafficking
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6931-9_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6929-6, 978-1-4939-6931-9
|
| Authors |
Benson, Robert A., Brewer, James M., Garside, Paul, Robert A. Benson, James M. Brewer, Paul Garside |
| Editors |
George Edward Rainger, Helen M. Mcgettrick |
| Abstract |
Advanced cellular tracking and imaging techniques allow the dynamic nature of immune responses to be studied in detail and in a physiological context. Here we describe two methods applying multiphoton laser scanning microscopy to the visualization and tracking of fluorescently labeled CD4(+) T cells and dendritic cells (DCs) within the complex lymph node (LN) environment. Ex vivo imaging of LNs allows the study of cell populations without the need for skilled surgical techniques while providing comparable data. While more technically demanding, intravital imaging of the popliteal LN allows aspects of T cell/DC responses to be studied in the context of an intact lymph and blood supply. We also describe methods to aid the acquisition of time series data suitable for cellular tracking, providing a quantitative approach to real-time analysis of DC and T cell LN responses. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 33% |
| Professor | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Medicine and Dentistry | 1 | 17% |
| Unknown | 3 | 50% |