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Recombinant Virus Vaccines

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Attention for Chapter 3: Fowl Adenovirus-Based Vaccine Platform
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Chapter title
Fowl Adenovirus-Based Vaccine Platform
Chapter number 3
Book title
Recombinant Virus Vaccines
Published in
Methods in molecular biology, April 2017
DOI 10.1007/978-1-4939-6869-5_3
Pubmed ID
Book ISBNs
978-1-4939-6867-1, 978-1-4939-6869-5
Authors

Juan C. Corredor, Yanlong Pei, Éva Nagy

Editors

Maureen C. Ferran, Gary R. Skuse

Abstract

Nonpathogenic fowl adenoviruses (FAdVs) are amenable for engineering multivalent vaccine platforms due to large stretches of nonessential DNA sequences in their genomes. We describe the generation of FAdV-9-based vaccine platforms by targeted homologous recombination in an infectious clone (pPacFAdV-9 or wild type FAdmid) containing the entire viral genome in a cosmid vector. The viral DNA is subsequently released from the cosmid by restriction enzyme digestion followed by transfection in a chicken hepatoma cell line (CH-SAH). Virus is harvested, propagated, and verified for foreign gene expression.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 27%
Researcher 4 27%
Student > Ph. D. Student 2 13%
Student > Bachelor 1 7%
Student > Postgraduate 1 7%
Other 0 0%
Unknown 3 20%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 2 13%
Agricultural and Biological Sciences 2 13%
Unspecified 1 7%
Biochemistry, Genetics and Molecular Biology 1 7%
Computer Science 1 7%
Other 3 20%
Unknown 5 33%