| Chapter title |
Human Rhinovirus-A1 as an Expression Vector
|
|---|---|
| Chapter number | 11 |
| Book title |
Recombinant Virus Vaccines
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6869-5_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6867-1, 978-1-4939-6869-5
|
| Authors |
Tomusange, Khamis, Wijesundara, Danushka, Gowans, Eric James, Grubor-Bauk, Branka, Khamis Tomusange, Danushka Wijesundara, Eric James Gowans, Branka Grubor-Bauk |
| Editors |
Maureen C. Ferran, Gary R. Skuse |
| Abstract |
Expression vectors that are based on live human rhinoviruses (HRVs) are attractive, yet often overlooked in vaccine development due to their limited capacity for foreign gene inserts and poor genetic stability. This chapter describes a novel methodology to engineer a replication-competent genetically stable recombinant HRV (rHRV) without affecting viral replication capability. We have previously used these methods to generate live, genetically stable recombinant HRVs encoding HIV Gag and Tat proteins (rHRV-Gag-Tat), a potential mucosally targeted HIV vaccine. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 20% |
| Researcher | 1 | 20% |
| Lecturer | 1 | 20% |
| Other | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Agricultural and Biological Sciences | 1 | 20% |
| Sports and Recreations | 1 | 20% |
| Immunology and Microbiology | 1 | 20% |
| Unknown | 1 | 20% |