Chapter title |
The SWHEL System for High-Resolution Analysis of In Vivo Antigen-Specific T-Dependent B Cell Responses
|
---|---|
Chapter number | 9 |
Book title |
T follicular Helper Cells
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2498-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2497-4, 978-1-4939-2498-1
|
Authors |
R. Brink, D. Paus, K. Bourne, J. R. Hermes, S. Gardam, T. G. Phan, T. D. Chan |
Editors |
Marion Espéli, Michelle Linterman |
Abstract |
T cell-dependent B cell responses generate optimal antibodies to combat foreign antigens. Naïve B cells responding to antigen undergo a complex series of differentiation events and cell fate decisions to provide long-lived memory B cells and plasma cells. Historically, B cell biologists have been challenged by the task of investigating rare antigen-specific B cells in an in vivo setting such that their interactions with antigen, regulation and migration may be accurately tracked. We have developed the SWHEL experimental system capable of accurately monitoring B cells that interact with a protein antigen and then subsequently undergo isotype switching, somatic hypermutation, and affinity maturation within germinal centers (GC) to generate high-affinity antibodies. Here we provide a comprehensive description of the procedures involved in establishing and using the SWHEL system to assess B cell responses to a foreign antigen. This system can provide a valuable measure of the functional capabilities of T follicular helper cells, whose role is ultimately to support and shape long-term humoral immunity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 37% |
Student > Bachelor | 4 | 21% |
Researcher | 3 | 16% |
Professor > Associate Professor | 1 | 5% |
Unknown | 4 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 7 | 37% |
Agricultural and Biological Sciences | 5 | 26% |
Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Engineering | 1 | 5% |
Unknown | 5 | 26% |