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Mendeley readers
| Chapter title |
Triggering Positive Selection of Germinal Center B Cells by Antigen Targeting to DEC-205
|
|---|---|
| Chapter number | 10 |
| Book title |
T follicular Helper Cells
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2498-1_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2497-4, 978-1-4939-2498-1
|
| Authors |
Giulia Pasqual, Alessandro Angelini, Gabriel D. Victora |
| Editors |
Marion Espéli, Michelle Linterman |
| Abstract |
Germinal centers (GCs) are the site of maturation of antibody affinity and are thus of key importance to humoral immunity. The study of B-cell dynamics and selection within the GC has been hampered by the limited number of techniques available to manipulate GC output in vivo. Here, we describe an approach to trigger positive selection of B cells in vivo by targeting antigen specifically to a subpopulation of GC B cells via the surface lectin DEC-205 and forcing their interaction with T follicular helper cells. Targeted GC B cells can then be followed over time as they progress through the stages of positive selection. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 40% |
| Researcher | 3 | 15% |
| Professor > Associate Professor | 2 | 10% |
| Professor | 1 | 5% |
| Student > Master | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 4 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 7 | 35% |
| Biochemistry, Genetics and Molecular Biology | 4 | 20% |
| Agricultural and Biological Sciences | 4 | 20% |
| Medicine and Dentistry | 1 | 5% |
| Unknown | 4 | 20% |