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T follicular Helper Cells

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Cover of 'T follicular Helper Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Identification of mouse T follicular helper cells by flow cytometry.
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    Chapter 2 Identification of follicular T helper cells in tissue sections.
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    Chapter 3 Tracking early T follicular helper cell differentiation in vivo.
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    Chapter 4 Tracking by Flow Cytometry Antigen-Specific Follicular Helper T Cells in Wild-Type Animals After Protein Vaccination
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    Chapter 5 Retroviral Vector Expression in TCR Transgenic CD4(+) T Cells.
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    Chapter 6 Two-photon microscopy for imaging germinal centers and T follicular helper cells.
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    Chapter 7 In Vivo Induction of T-Follicular Helper Cells by Modulation of Regulatory T Cell Function
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    Chapter 8 Assessing T Follicular Helper Cell Function In Vivo: Antigen-Specific B Cell Response to Hapten and Affinity Maturation
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    Chapter 9 The SWHEL System for High-Resolution Analysis of In Vivo Antigen-Specific T-Dependent B Cell Responses
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    Chapter 10 Triggering Positive Selection of Germinal Center B Cells by Antigen Targeting to DEC-205
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    Chapter 11 Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry.
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    Chapter 12 Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry
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    Chapter 13 In Vitro Assay to Sensitively Measure TFR Suppressive Capacity and TFH Stimulation of B Cell Responses.
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    Chapter 14 Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells
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    Chapter 15 Human tfh and tfr cells: identification and assessment of their migration potential.
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    Chapter 16 Analysis of human blood memory T follicular helper subsets.
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    Chapter 17 Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood
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    Chapter 18 Quantifying Helper Cell Function of Human TFH Cells In Vitro.
Attention for Chapter 12: Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry
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Chapter title
Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry
Chapter number 12
Book title
T follicular Helper Cells
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2498-1_12
Pubmed ID
Book ISBNs
978-1-4939-2497-4, 978-1-4939-2498-1
Authors

Ana Raquel Maceiras, Luis Graca

Editors

Marion Espéli, Michelle Linterman

Abstract

Flow cytometry is a technology that allows multiparametric analysis of individual cells. As a consequence, it is among the most commonly used tools for the study of immune cells. It is useful both for the study of ex vivo cell populations isolated from experimental animals or human tissue and for characterizing the phenotype of cultured cells. The phenotypic analysis is based on antibodies associated to different fluorophores that specifically bind to key molecules. Genetically modified mouse strains that express a reporter gene under the control of a promoter of interest offer an important alternative for the staining of intracellular molecules without the need to permeabilize the cell membrane. In this chapter, we describe how Foxp3(+) follicular regulatory T (Tfr) cells, a population of regulatory T (Treg) cells related to T follicular helper (Tfh) cells and involved in the regulation of germinal centers (GC), can be identified by flow cytometry.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 37%
Professor > Associate Professor 3 16%
Student > Ph. D. Student 3 16%
Librarian 1 5%
Other 1 5%
Other 1 5%
Unknown 3 16%
Readers by discipline Count As %
Immunology and Microbiology 10 53%
Biochemistry, Genetics and Molecular Biology 2 11%
Medicine and Dentistry 2 11%
Social Sciences 1 5%
Engineering 1 5%
Other 0 0%
Unknown 3 16%