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Stem Cell Banking

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Attention for Chapter 9: Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium
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Chapter title
Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium
Chapter number 9
Book title
Stem Cell Banking
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6921-0_9
Pubmed ID
Book ISBNs
978-1-4939-6919-7, 978-1-4939-6921-0
Authors

Guokai Chen, Mahendra Rao

Editors

Jeremy M. Crook, Tenneille E. Ludwig

Abstract

Human somatic cells can be reprogrammed by defined factors to induced pluripotent stem cells (iPSCs). Importantly, the quality of iPSCs could impact the potential of these cells in basic and clinic research. Here, we describe a method to reprogram human fibroblast cells with Sendai virus in chemically defined conditions, to generate iPSCs that are integration-free and suitable for research and translational applications.

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Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Unknown 2 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Unknown 1 50%