| Chapter title |
Cryobanking Pluripotent Stem Cells
|
|---|---|
| Chapter number | 11 |
| Book title |
Stem Cell Banking
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6921-0_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6919-7, 978-1-4939-6921-0
|
| Authors |
Jeremy M. Crook, Eva Tomaskovic-Crook, Tenneille E. Ludwig, Crook, Jeremy M., Tomaskovic-Crook, Eva, Ludwig, Tenneille E. |
| Editors |
Jeremy M. Crook, Tenneille E. Ludwig |
| Abstract |
Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryopreservation, suitable for routine application, high-quality research, and adaptable for clinical compliance. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 22% |
| Student > Postgraduate | 2 | 22% |
| Professor > Associate Professor | 1 | 11% |
| Unspecified | 1 | 11% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 22% |
| Veterinary Science and Veterinary Medicine | 1 | 11% |
| Unspecified | 1 | 11% |
| Neuroscience | 1 | 11% |
| Chemistry | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 2 | 22% |