Chapter title |
Isolation, Culture, and Expansion of Mesenchymal Stem Cells
|
---|---|
Chapter number | 13 |
Book title |
Stem Cell Banking
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6921-0_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6919-7, 978-1-4939-6921-0
|
Authors |
Izaskun Ferrin, Izaskun Beloqui, Lorea Zabaleta, Juan M. Salcedo, Cesar Trigueros, Angel G. Martin |
Editors |
Jeremy M. Crook, Tenneille E. Ludwig |
Abstract |
Mesenchymal stem cells (MSCs), together with hematopoietic stem cells (HSCs), are the most frequently used cell type for cell-based therapeutics. As for other cell types intended for research and translational use, it is important to establish correctly typed cell lines from human tissue donations. Here, we describe methods for isolating, culturing, and identifying MSCs from various tissues obtained through human tissue donation. The methods have been used in the context of a biobank, prepared as standard operating procedures (SOPs), ensuring traceability and reproducibility of cell production. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 6 | 14% |
Student > Master | 6 | 14% |
Researcher | 5 | 12% |
Student > Ph. D. Student | 3 | 7% |
Other | 2 | 5% |
Other | 2 | 5% |
Unknown | 19 | 44% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 10 | 23% |
Medicine and Dentistry | 6 | 14% |
Immunology and Microbiology | 2 | 5% |
Environmental Science | 1 | 2% |
Agricultural and Biological Sciences | 1 | 2% |
Other | 3 | 7% |
Unknown | 20 | 47% |