Chapter title |
Genome Editing in Human Pluripotent Stem Cells
|
---|---|
Chapter number | 12 |
Book title |
Stem Cell Banking
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6921-0_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6919-7, 978-1-4939-6921-0
|
Authors |
Carlson-Stevermer, Jared, Saha, Krishanu, Jared Carlson-Stevermer, Krishanu Saha |
Editors |
Jeremy M. Crook, Tenneille E. Ludwig |
Abstract |
Genome editing in human pluripotent stem cells (hPSCs) enables the generation of reporter lines and knockout cell lines. Zinc finger nucleases, transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9 technology have recently increased the efficiency of proper gene editing by creating double strand breaks (DSB) at defined sequences in the human genome. These systems typically use plasmids to transiently transcribe nucleases within the cell. Here, we describe the process for preparing hPSCs for transient expression of nucleases via electroporation and subsequent analysis to create genetically modified stem cell lines. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 33% |
Italy | 1 | 17% |
India | 1 | 17% |
Unknown | 2 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 5 | 83% |
Scientists | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | 4% |
United Kingdom | 2 | 1% |
Netherlands | 1 | <1% |
Australia | 1 | <1% |
Switzerland | 1 | <1% |
Germany | 1 | <1% |
Brazil | 1 | <1% |
China | 1 | <1% |
Mexico | 1 | <1% |
Other | 0 | 0% |
Unknown | 141 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 47 | 30% |
Student > Ph. D. Student | 32 | 21% |
Student > Bachelor | 13 | 8% |
Student > Master | 13 | 8% |
Professor > Associate Professor | 11 | 7% |
Other | 25 | 16% |
Unknown | 15 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 80 | 51% |
Biochemistry, Genetics and Molecular Biology | 28 | 18% |
Medicine and Dentistry | 11 | 7% |
Neuroscience | 8 | 5% |
Chemistry | 3 | 2% |
Other | 7 | 4% |
Unknown | 19 | 12% |