Chapter title |
Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, During Bacterial Infection in Arabidopsis
|
---|---|
Chapter number | 16 |
Book title |
Plant Pattern Recognition Receptors
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6859-6_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6858-9, 978-1-4939-6859-6
|
Authors |
Lin Jin, David M. Mackey |
Editors |
Libo Shan, Ping He |
Abstract |
The plant cell wall responds dynamically during interaction with various pathogens. Upon recognition of "nonself" components, plant cells deploy a variety of immune responses including cell wall fortification. Callose, a β-(1, 3)-D-glucan polymer, is a component of the material deposited at the site of infection between the plasma membrane and the preexisting cell wall that is hypothesized to serve as a physical barrier and platform for directed antimicrobial compound deposition. The defense-associated function of callose deposition is supported by its induction during pathogen-associated molecular patterns (PAMP)-triggered immunity (PTI) and its inhibition by defense suppressing virulence effectors. Thus, callose deposition is a commonly monitored read-out in plant defense. This protocol describes the use of aniline blue staining and fluorescent microscopy to measure callose deposition in bacteria-infected or elicitor-challenged Arabidopsis leaf tissues. |
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