Plant Pattern Recognition Receptors
Springer New York
Chapter title |
Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants
|
---|---|
Chapter number | 24 |
Book title |
Plant Pattern Recognition Receptors
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6859-6_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6858-9, 978-1-4939-6859-6
|
Authors |
Jian Hua, Shuai Wang, Qi Sun |
Editors |
Libo Shan, Ping He |
Abstract |
Here we describe a method of gene mapping and cloning of chemical induced mutations using next-generation sequencing on their backcrossed segregants. This method utilizes polymorphisms induced by chemical mutagens for mapping and therefore does not require outcrossing to a different background or a large segregating population. It can be used to clone causal mutations generated in various accessions or mutant backgrounds for dissecting complex processes such as pattern recognition receptor pathways in plant immunity. |
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 57% |
Researcher | 2 | 29% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 57% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Environmental Science | 1 | 14% |
Unknown | 1 | 14% |