Chapter title |
LeEIX2 Interactors’ Analysis and EIX-Mediated Responses Measurement
|
---|---|
Chapter number | 13 |
Book title |
Plant Pattern Recognition Receptors
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6859-6_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6858-9, 978-1-4939-6859-6
|
Authors |
Meirav Leibman-Markus, Silvia Schuster, Adi Avni |
Editors |
Libo Shan, Ping He |
Abstract |
Plant-pathogen interactions involve a large number of wide regulatory systems, necessary for plant defense responses against pathogen attack. The fungal protein ethylene-inducing xylanase (EIX) elicits defense responses in specific cultivars of tobacco and tomato. The response to EIX is controlled by a single locus encoding for LeEIX2, a leucine-rich-repeat receptor-like-protein (LRR-RLP). As an RLP, LeEIX2 does not possess an obvious cytoplasmic signaling moiety such as a kinase domain. To study LeEIX2 mode of action, it is essential to identify the potential interactors involved after EIX perception. Here, we describe the in vivo co-IP methodology used for protein interaction verification and ethylene and ROS (reactive oxygen species) measurements used for physiological effects assessment. |
Mendeley readers
Geographical breakdown
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 7 | 33% |
Student > Ph. D. Student | 5 | 24% |
Professor | 2 | 10% |
Student > Master | 2 | 10% |
Student > Bachelor | 1 | 5% |
Other | 1 | 5% |
Unknown | 3 | 14% |
Readers by discipline | Count | As % |
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Unknown | 4 | 19% |