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Mendeley readers
Chapter title |
Chitin and Stress Induced Protein Kinase Activation
|
---|---|
Chapter number | 15 |
Book title |
Plant Pattern Recognition Receptors
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Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6859-6_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6858-9, 978-1-4939-6859-6
|
Authors |
Chandra Kenchappa, Raquel Azevedo da Silva, Simon Bressendorff, Sabrina Stanimirovic, Jakob Olsen, Morten Petersen, John Mundy |
Editors |
Libo Shan, Ping He |
Abstract |
The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 1 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Environmental Science | 1 | 100% |