Chapter title |
Analysis of PAMP-Triggered ROS Burst in Plant Immunity
|
---|---|
Chapter number | 11 |
Book title |
Plant Pattern Recognition Receptors
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6859-6_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6858-9, 978-1-4939-6859-6
|
Authors |
Yuying Sang, Alberto P. Macho |
Editors |
Libo Shan, Ping He |
Abstract |
The plant perception of pathogen-associated molecular patterns triggers a plethora of cellular immune responses. One of these responses is a rapid and transient burst of reactive oxygen species (ROS) mediated by plasma membrane-localized NADPH oxidases. The ROS burst requires a functional receptor complex and the contribution of several additional regulatory components. In laboratory conditions, the ROS burst can be detected a few minutes after the treatment with an immunogenic microbial elicitor. For these reasons, the elicitor-triggered ROS burst has been often exploited as readout to probe the contribution of plant components to early immune responses. Here, we describe a detailed protocol for the measurement of elicitor-triggered ROS burst in a simple, fast, and easy manner. |
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