Chapter title |
MAPK Assays in Arabidopsis MAMP-PRR Signal Transduction
|
---|---|
Chapter number | 12 |
Book title |
Plant Pattern Recognition Receptors
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6859-6_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6858-9, 978-1-4939-6859-6
|
Authors |
Hoo Sun Chung, Jen Sheen |
Editors |
Libo Shan, Ping He |
Abstract |
Activation of MAPK (Mitogen-Activated Protein Kinase) cascades after MAMP (Microbe-Associated Molecular Pattern) perception through PRR (Pattern Recognition Receptor) is one of the first conserved responses when plants encounter microbial organisms. Phosphorylation of various cellular factors in the MAMP-PRR pathway by MAPK cascades is critical for broad-spectrum plant innate immunity. Measurement of MAPK activation and identification of MAPK phosphorylation targets in the MAMP-PRR signal transduction pathway are essential to understand how plants reprogram their cellular processes to cope with unfavorable microbial attack. Here, we describe detailed protocols of three assays measuring MAPK activity after MAMP perception: (1) immune-blotting analysis with anti-phospho ERK1/2 antibody; (2) in-gel kinase assay using a general substrate myelin basic protein (MBP); (3) an in vitro kinase assay to evaluate phosphorylation of MAPK substrate candidates during MAMP-PRR signaling based on a protoplast expression system. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 15% |
Researcher | 2 | 10% |
Student > Ph. D. Student | 2 | 10% |
Student > Bachelor | 1 | 5% |
Unspecified | 1 | 5% |
Other | 2 | 10% |
Unknown | 9 | 45% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 30% |
Biochemistry, Genetics and Molecular Biology | 4 | 20% |
Unspecified | 1 | 5% |
Unknown | 9 | 45% |