Chapter title |
Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate
|
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Chapter number | 9 |
Book title |
Hypertension
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6625-7_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6623-3, 978-1-4939-6625-7
|
Authors |
Srinivas Sriramula, Kim Brint Pedersen, Huijing Xia, Eric Lazartigues |
Editors |
Rhian M. Touyz, Ernesto L. Schiffrin |
Abstract |
Angiotensin-converting enzyme 2 (ACE2) is a component of the renin-angiotensin system (RAS) which plays an important role in the regulation of blood pressure and volume homeostasis. Accumulating evidence shows alterations in ACE2 expression and activity in several hypertensive animal models, as well as in patients with hypertension. In order to assess the role of brain ACE2 in hypertension, a specific ACE2 assay is required. Based on a quenched fluorescent substrate, we describe an easy-to-use method for determining ACE2 activity in brain tissue and cerebrospinal fluid. The method can further be adapted for other tissues, plasma, cell extracts, and cell culture supernatants. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 29% |
Student > Ph. D. Student | 1 | 14% |
Other | 1 | 14% |
Student > Postgraduate | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
Nursing and Health Professions | 1 | 14% |
Medicine and Dentistry | 1 | 14% |
Unknown | 2 | 29% |