Chapter title |
Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos.
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Chapter number | 487 |
Book title |
Embryo Models In Vitro
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Published in |
Methods in molecular biology, June 2023
|
DOI | 10.1007/7651_2023_487 |
Pubmed ID | |
Book ISBNs |
978-1-07-163685-5, 978-1-07-163686-2
|
Authors |
Biondic, Savana, Zhao, Cheng, Hagemann-Jensen, Michael, Russell, Stewart J, Vandal, Katherine, Canizo, Jesica, Librach, Clifford L, Petropoulos, Sophie, Russell, Stewart J., Librach, Clifford L. |
Abstract |
The development of single-cell multiomics has provided the ability to systematically investigate cellular diversity and heterogeneity in different biological systems via comprehensive delineations of individual cellular states. Single-cell RNA sequencing in particular has served as a powerful tool to the study of the molecular circuitries underlying preimplantation embryonic development in both the mouse and human. Here we describe a method to elucidate the cellular dynamics of the embryo further by performing both single-cell RNA sequencing (Smart-Seq2) and single-cell small non-coding RNA sequencing (Small-Seq) on the same individual embryonic cell. |
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