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Embryo Models In Vitro

Overview of attention for book
Cover of 'Embryo Models In Vitro'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 463 Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models
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    Chapter 464 Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology
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    Chapter 465 Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL
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    Chapter 466 Human Pre-gastrulation Embryo Culture in 3D Condition
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    Chapter 469 Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast
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    Chapter 470 Stem Cell-Derived Microfluidic Amniotic Sac Embryoid ( μ PASE)
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    Chapter 471 Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.
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    Chapter 483 Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells
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    Chapter 485 Generation of Human Blastoids from Naive Pluripotent Stem Cells
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    Chapter 486 Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts
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    Chapter 487 Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos.
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    Chapter 488 Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence
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    Chapter 489 ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis
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    Chapter 490 Whole-Mount RNA, Single-Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos.
  16. Altmetric Badge
    Chapter 491 Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond
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    Chapter 492 Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas
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    Chapter 493 Generation of Stem Cell-Based Mouse Embryo-Like Structures
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    Chapter 494 Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics
  20. Altmetric Badge
    Chapter 496 Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells.
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    Chapter 507 Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells.
Attention for Chapter 490: Whole-Mount RNA, Single-Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos.
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Chapter title
Whole-Mount RNA, Single-Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos.
Chapter number 490
Book title
Embryo Models In Vitro
Published in
Methods in molecular biology, June 2023
DOI 10.1007/7651_2023_490
Pubmed ID
Book ISBNs
978-1-07-163685-5, 978-1-07-163686-2
Authors

Canizo, Jesica, Vandal, Katherine, Biondic, Savana, Petropoulos, Sophie

Abstract

Fluorescence in situ hybridization (FISH) provides a valuable tool for studying the spatial localization of and expression level of genes and cell function in diverse biological contexts. In this chapter, we describe a protocol for the simultaneous detection of RNA (including single-molecule (sm)RNA) and DNA in mammalian embryos using FISH. RNA FISH is a technique that enables the detection and visualization of specific RNA molecules within cells. With advancements in technology, the sensitivity and specificity of RNA FISH has been improved to allow the detection of individual mRNA molecules. Both RNA and smRNA are detected using a set of fluorescent-labeled probes, which are complementary to a specific nucleotide sequence corresponding to the gene of interest. These probes hybridize to the target RNA molecules, enabling the simultaneous detection of multiple RNAs within the same cell or tissue. DNA FISH is performed using probes directed at the DNA sequence to detect the genome region of interest. In this chapter, we provide a protocol to process mammalian embryos for FISH with probe examples specifically for studying X-Chromosome activity. By utilizing other probe designs, this protocol can be adapted for the visualization and quantification of other genes and chromosomal regions of interest.

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Geographical breakdown

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Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 50%
Unknown 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2023.
All research outputs
#21,284,466
of 23,910,532 outputs
Outputs from Methods in molecular biology
#10,285
of 13,500 outputs
Outputs of similar age
#150,577
of 193,271 outputs
Outputs of similar age from Methods in molecular biology
#43
of 84 outputs
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