Chapter title |
Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.
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Chapter number | 471 |
Book title |
Embryo Models In Vitro
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Published in |
Methods in molecular biology, February 2023
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DOI | 10.1007/7651_2022_471 |
Pubmed ID | |
Book ISBNs |
978-1-07-163685-5, 978-1-07-163686-2
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Authors |
Luo, Yu-Xin, Yu, Yang |
Abstract |
Stem cell-derived embryos in vitro allow the exploration of the very early stages of human embryogenesis in vitro and are thus promising for widespread applications in developmental biology, related developmental disease modeling, and drug discovery. Several cell resources have been utilized, with different efficiencies and methods for generating human blastoids, a structure similar to natural blastocysts. Human EPS cells were reported to contribute to the embryonic and extraembryonic lineages and therefore can be a practical and efficient cell resource for constructing human blastoids. Here, we developed a three-dimensional, two-step induction system for generating human blastoids using human EPS cells. According to morphological and transcriptomic analysis, EPS-blastoids recapitulate the key developmental processes and cell lineages of human blastocysts. Moreover, in vitro extended culture for 8 and 10 days of EPS-blastoids can result in postimplantation embryonic structures. In this chapter, we describe a protocol that covers the generation, maintenance, and developmental phenocopying of human EPS blastoids. |
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Members of the public | 1 | 100% |