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Mendeley readers
Chapter title |
Expression and Purification of SH2 Domains Using Baculovirus Expression System
|
---|---|
Chapter number | 11 |
Book title |
SH2 Domains
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6762-9_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6760-5, 978-1-4939-6762-9
|
Authors |
Mari Ogiue-Ikeda, Kazuya Machida |
Editors |
Kazuya Machida, Bernard A. Liu |
Abstract |
Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble and functionally active proteins. We describe a protocol for cloning and purification of GST-tagged SH2 domains using the Bac-to-Bac baculovirus expression system. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 25% |
Professor > Associate Professor | 1 | 25% |
Other | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Computer Science | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |
Chemistry | 1 | 25% |
Unknown | 1 | 25% |