Chapter title |
Purification of SOCS (Suppressor of Cytokine Signaling) SH2 Domains for Structural and Functional Studies
|
---|---|
Chapter number | 10 |
Book title |
SH2 Domains
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6762-9_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6760-5, 978-1-4939-6762-9
|
Authors |
Nicholas P. D. Liau, Artem Laktyushin, Jeffrey J. Babon, Liau, Nicholas P. D., Laktyushin, Artem, Babon, Jeffrey J. |
Editors |
Kazuya Machida, Bernard A. Liu |
Abstract |
Src Homology 2 (SH2) domains are protein domains which have a high binding affinity for specific amino acid sequences containing a phosphorylated tyrosine residue. The Suppressors of Cytokine Signaling (SOCS) proteins use an SH2 domain to bind to components of certain cytokine signaling pathways to downregulate the signaling cascade. The recombinantly produced SH2 domains of various SOCS proteins have been used to undertake structural and functional studies elucidating the method of how such targeting occurs. Here, we describe the protocol for the recombinant production and purification of SOCS SH2 domains, with an emphasis on SOCS3. |
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