Genotype Phenotype Coupling
Springer US
Chapter title |
Streamlined Data Analysis Pipeline for Deep Sequence-Coupled Biopanning Identification of Pathogen-Specific Antibody Responses in Serum.
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Chapter number | 23 |
Book title |
Genotype Phenotype Coupling
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Published in |
Methods in molecular biology, January 2023
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DOI | 10.1007/978-1-0716-3279-6_23 |
Pubmed ID | |
Book ISBNs |
978-1-07-163278-9, 978-1-07-163279-6
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Authors |
Leo, Javier, Core, Susan B, Frietze, Kathryn M, Core, Susan B., Frietze, Kathryn M., Javier Leo, Susan B. Core, Kathryn M. Frietze |
Abstract |
Deep sequence-coupled biopanning (DSCB) is a powerful tool that couples affinity selection of a bacteriophage MS2 virus-like particle peptide display platform with deep sequencing. While this approach has been used successfully to investigate pathogen-specific antibody responses in human sera, data analysis is time-consuming and complicated. Here, we describe a streamlined data analysis method for DSCB using MATLAB, expanding the potential for this approach to be deployed rapidly and consistently. |
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