Chapter title |
Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions
|
---|---|
Chapter number | 10 |
Book title |
Biobanking and Cryopreservation of Stem Cells
|
Published in |
Advances in experimental medicine and biology, November 2016
|
DOI | 10.1007/978-3-319-45457-3_10 |
Pubmed ID | |
Book ISBNs |
978-3-31-945455-9, 978-3-31-945457-3
|
Authors |
Marcela K. Preininger, Monalisa Singh, Chunhui Xu, Preininger, Marcela K., Singh, Monalisa, Xu, Chunhui |
Editors |
Feridoun Karimi-Busheri, Michael Weinfeld |
Abstract |
In recent years, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as a vital cell source for in vitro modeling of genetic cardiovascular disorders, drug screening, and in vivo cardiac regeneration research. Looking forward, the ability to efficiently cryopreserve hPSC-CMs without compromising their normal biochemical and physiologic functions will dramatically facilitate their various biomedical applications. Although working protocols for freezing, storing, and thawing hPSC-CMs have been established, the question remains as to whether they are optimal. In this chapter, we discuss our current understanding of cryopreservation appertaining to hPSC-CMs, and proffer key questions regarding the mechanical, contractile, and regenerative properties of cryopreserved hPSC-CMs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 81 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 16 | 20% |
Student > Bachelor | 11 | 14% |
Student > Master | 10 | 12% |
Student > Doctoral Student | 7 | 9% |
Researcher | 6 | 7% |
Other | 12 | 15% |
Unknown | 19 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 22 | 27% |
Engineering | 12 | 15% |
Agricultural and Biological Sciences | 9 | 11% |
Medicine and Dentistry | 6 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
Other | 9 | 11% |
Unknown | 21 | 26% |