Chapter title |
Preservation of Ocular Epithelial Limbal Stem Cells: The New Frontier in Regenerative Medicine
|
---|---|
Chapter number | 15 |
Book title |
Biobanking and Cryopreservation of Stem Cells
|
Published in |
Advances in experimental medicine and biology, November 2016
|
DOI | 10.1007/978-3-319-45457-3_15 |
Pubmed ID | |
Book ISBNs |
978-3-31-945455-9, 978-3-31-945457-3
|
Authors |
Zala Lužnik, Marina Bertolin, Claudia Breda, Barbara Ferrari, Vanessa Barbaro, Petra Schollmayer, Stefano Ferrari |
Editors |
Feridoun Karimi-Busheri, Michael Weinfeld |
Abstract |
Significant advances have been made in the field of ocular regenerative medicine. Promising stem cell-based therapeutic strategies have been translated into the clinical practice over the last few decades. These new stem cell-based therapies offer the possibility of permanently restoring corneal epithelium in patients with severe disabling and blinding ocular surface disease. The European Union has already classified stem cell-based therapies as "medicinal products". Therefore, manipulation is strictly regulated according to the defined conditions of good manufacturing practice, with the production of stem cell therapeutics at only accredited production sites authorized by the national regulatory agencies. In this regard, as first medical products are licensed for commercial use in Europe enabling a more widespread access to a stem cell-based therapy, the need for safe, validated and reproducible techniques for ex vivo cultured tissue preservation and distribution are coming to the forefront of research. However, these provide various new challenges for biobanking industry such as the retention of viability, good functionality of stem cells and sterility issues. This chapter provides an overview of the current advances in the field of corneal/limbal epithelial stem cell culture preservation techniques using either hypothermic storage or cryopreservation methods, that were used in different culturing steps (from stem cell isolation to the ex vivo epithelial graft preparation), with the reported impact on the post-thawing product recovery. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 29% |
Student > Master | 2 | 12% |
Researcher | 2 | 12% |
Other | 1 | 6% |
Professor > Associate Professor | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 6 | 35% |
Business, Management and Accounting | 1 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Engineering | 1 | 6% |
Other | 0 | 0% |
Unknown | 7 | 41% |