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Biobanking and Cryopreservation of Stem Cells

Overview of attention for book
Biobanking and Cryopreservation of Stem Cells
Springer International Publishing

Table of Contents

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    Book Overview
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    Chapter 1 Key Issues Related to Cryopreservation and Storage of Stem Cells and Cancer Stem Cells: Protecting Biological Integrity
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    Chapter 2 Cryopreservation: Evolution of Molecular Based Strategies
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    Chapter 3 Fundamental Principles of Stem Cell Banking
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    Chapter 4 Biobanking: An Important Resource for Precision Medicine in Glioblastoma
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    Chapter 5 Slow Cooling Cryopreservation Optimized to Human Pluripotent Stem Cells
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    Chapter 6 Cryopreservation in Closed Bag Systems as an Alternative to Clean Rooms for Preparations of Peripheral Blood Stem Cells
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    Chapter 7 Cryopreserved or Fresh Mesenchymal Stromal Cells: Only a Matter of Taste or Key to Unleash the Full Clinical Potential of MSC Therapy?
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    Chapter 8 Biobanking of Human Mesenchymal Stem Cells: Future Strategy to Facilitate Clinical Applications
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    Chapter 9 Menstrual Blood-Derived Stem Cells: In Vitro and In Vivo Characterization of Functional Effects
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    Chapter 10 Cryopreservation of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Strategies, Challenges, and Future Directions
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    Chapter 11 Cryopreserved Adipose Tissue-Derived Stromal/Stem Cells: Potential for Applications in Clinic and Therapy
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    Chapter 12 Banking of Adipose- and Cord Tissue-Derived Stem Cells: Technical and Regulatory Issues
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    Chapter 13 Mature Oocyte Cryopreservation for Fertility Preservation
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    Chapter 14 Stem Cell Banking and Its Impact on Cardiac Regenerative Medicine
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    Chapter 15 Preservation of Ocular Epithelial Limbal Stem Cells: The New Frontier in Regenerative Medicine
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    Chapter 16 Cryopreservation of Hair-Follicle Associated Pluripotent (HAP) Stem Cells Maintains Differentiation and Hair-Growth Potential
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    Chapter 17 Cryopreservation and Banking of Dental Stem Cells
Attention for Chapter 5: Slow Cooling Cryopreservation Optimized to Human Pluripotent Stem Cells
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Chapter title
Slow Cooling Cryopreservation Optimized to Human Pluripotent Stem Cells
Chapter number 5
Book title
Biobanking and Cryopreservation of Stem Cells
Published in
Advances in experimental medicine and biology, November 2016
DOI 10.1007/978-3-319-45457-3_5
Pubmed ID
Book ISBNs
978-3-31-945455-9, 978-3-31-945457-3
Authors

Takamichi Miyazaki, Hirofumi Suemori

Editors

Feridoun Karimi-Busheri, Michael Weinfeld

Abstract

Human pluripotent stem cells (hPSCs) have the potential for unlimited expansion and differentiation into cells that form all three germ layers. Cryopreservation is one of the key processes for successful applications of hPSCs, because it allows semi-permanent preservation of cells and their easy transportation. Most animal cell lines, including mouse embryonic stem cells, are standardly cryopreserved by slow cooling; however, hPSCs have been difficult to preserve and their cell viability has been extremely low whenever cryopreservation has been attempted.Here, we investigate the reasons for failure of slow cooling in hPSC cryopreservation. Cryopreservation involves a series of steps and is not a straightforward process. Cells may die due to various reasons during cryopreservation. Indeed, hPSCs preserved by traditional methods often suffer necrosis during the freeze-thawing stages, and the colony state of hPSCs prior to cryopreservation is a major factor contributing to cell death.It has now become possible to cryopreserve hPSCs using conventional cryopreservation methods without any specific equipment. This review summarizes the advances in this area and discusses the optimization of slow cooling cryopreservation for hPSC storage.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 16%
Student > Ph. D. Student 3 16%
Researcher 3 16%
Student > Master 2 11%
Professor > Associate Professor 1 5%
Other 0 0%
Unknown 7 37%
Readers by discipline Count As %
Engineering 4 21%
Medicine and Dentistry 2 11%
Agricultural and Biological Sciences 1 5%
Biochemistry, Genetics and Molecular Biology 1 5%
Chemistry 1 5%
Other 1 5%
Unknown 9 47%