| Chapter title |
ChIP-seq Analysis of Condensin Complex in Cultured Mammalian Cells.
|
|---|---|
| Chapter number | 16 |
| Book title |
Cohesin and Condensin
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6545-8_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6543-4, 978-1-4939-6545-8
|
| Authors |
Toyonori Sakata, Katsuhiko Shirahige, Takashi Sutani |
| Editors |
Kyoko Yokomori, Katsuhiko Shirahige |
| Abstract |
ChIP-seq, or chromatin immunoprecipitation combined with massively parallel DNA sequencing, is a powerful technique to investigate in vivo protein-DNA interactions on a genome-wide scale at high resolution. Here we describe a ChIP-seq protocol optimized for analysis of condensin I complex on human mitotic chromosomes. The protocol includes procedures of intensive cell fixation by two cross-linking reagents and thorough chromatin shearing by nuclease and sonication treatments, both of which contribute to improving the signal-to-noise ratio of condensin I ChIP-seq profiles. The optimized protocol may also be helpful to explore chromosomal binding sites of other "hard-to-see" proteins by ChIP-seq. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 38% |
| Student > Ph. D. Student | 4 | 25% |
| Student > Bachelor | 1 | 6% |
| Professor | 1 | 6% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 50% |
| Agricultural and Biological Sciences | 4 | 25% |
| Computer Science | 1 | 6% |
| Environmental Science | 1 | 6% |
| Unknown | 2 | 13% |