Chapter title |
Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl.
|
---|---|
Chapter number | 3 |
Book title |
Cohesin and Condensin
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6545-8_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6543-4, 978-1-4939-6545-8
|
Authors |
Ge Zheng, Zhuqing Ouyang, Hongtao Yu |
Editors |
Kyoko Yokomori, Katsuhiko Shirahige |
Abstract |
During the cell cycle, duplicated sister chromatids become physically connected during S phase through a process called sister-chromatid cohesion. Cohesion is terminated during the metaphase-to-anaphase transition to trigger sister-chromatid segregation. The establishment and dissolution of cohesion are highly regulated by the cohesin complex and its multitude of regulators. In particular, the cohesin regulator Wapl promotes the release of cohesin from chromosomes during both interphase and mitosis. Here, we describe in vitro protein binding assays between Wapl and a cohesin subcomplex, and cellular assays in human cells that probe the functions of Wapl in cohesin release. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 21% |
Professor | 3 | 21% |
Researcher | 3 | 21% |
Student > Master | 2 | 14% |
Other | 1 | 7% |
Other | 0 | 0% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 50% |
Agricultural and Biological Sciences | 5 | 36% |
Unknown | 2 | 14% |