| Chapter title |
Detection of Cohesin SUMOylation In Vivo.
|
|---|---|
| Chapter number | 4 |
| Book title |
Cohesin and Condensin
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6545-8_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6543-4, 978-1-4939-6545-8
|
| Authors |
Marcelino Bermúdez-López, Luis Aragón |
| Editors |
Kyoko Yokomori, Katsuhiko Shirahige |
| Abstract |
Cohesin is a protein complex with key roles in chromosome biology, from chromatid segregation to DNA repair. Cohesin function is regulated by several posttranslational modifications, including phosphorylation, acetylation, ubiquitylation, and SUMOylation. Recent studies have shown that cohesin SUMOylation is essential for sister chromatid cohesion during normal cell cycle and in response to DNA damage. Posttranslational modification by the small ubiquitin-like modifier (SUMO) is a field in expansion, however, detecting SUMOylation can be challenging because the amount of modified substrates are usually low and de-conjugation during sample preparation often occurs. In this chapter we describe a method that can be adapted to different model organisms, and substrates to detect SUMOylation. We focus on cohesin and show that SUMOylation indeed occurs in most of the subunits of budding yeast cohesin. |
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Geographical breakdown
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|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Researcher | 2 | 29% |
| Professor | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Agricultural and Biological Sciences | 1 | 14% |
| Unknown | 1 | 14% |