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Chapter title |
A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.
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Chapter number | 9 |
Book title |
Cohesin and Condensin
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6545-8_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6543-4, 978-1-4939-6545-8
|
Authors |
Jinbo Fan, Hui Jin, Hong-Guo Yu |
Editors |
Kyoko Yokomori, Katsuhiko Shirahige |
Abstract |
In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a cis-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method described here provides an effective cytological approach for quantitative analysis of global gene expression in budding yeast meiosis. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 40% |
Student > Ph. D. Student | 1 | 20% |
Researcher | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 60% |
Agricultural and Biological Sciences | 1 | 20% |
Unknown | 1 | 20% |