Chapter title |
Synthetic Gene Design Using Codon Optimization On-Line (COOL).
|
---|---|
Chapter number | 2 |
Book title |
Synthetic DNA
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6343-0_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6341-6, 978-1-4939-6343-0
|
Authors |
Kai Yu, Kok Siong Ang, Dong-Yup Lee |
Editors |
Randall A. Hughes |
Abstract |
Codon optimization has been widely used for designing native or synthetic genes to enhance their expression in heterologous host organisms. We recently developed Codon Optimization On-Line (COOL) which is a web-based tool to provide multi-objective codon optimization functionality for synthetic gene design. COOL provides a simple and flexible interface for customizing codon optimization based on several design parameters such as individual codon usage, codon pairing, and codon adaptation index. User-defined sequences can also be compared against the COOL optimized ones to show the extent by which the user's sequences can be evaluated and further improved. The utility of COOL is demonstrated via a case study where the codon optimized sequence of an invertase enzyme is generated for the enhanced expression in E. coli. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Singapore | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
China | 1 | 4% |
Thailand | 1 | 4% |
Unknown | 24 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 31% |
Researcher | 7 | 27% |
Student > Master | 3 | 12% |
Professor | 1 | 4% |
Student > Doctoral Student | 1 | 4% |
Other | 1 | 4% |
Unknown | 5 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 31% |
Agricultural and Biological Sciences | 6 | 23% |
Immunology and Microbiology | 2 | 8% |
Chemical Engineering | 1 | 4% |
Computer Science | 1 | 4% |
Other | 1 | 4% |
Unknown | 7 | 27% |