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Synthetic DNA

Overview of attention for book
Cover of 'Synthetic DNA'

Table of Contents

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    Book Overview
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    Chapter 1 A Guide to Using STITCHER for Overlapping Assembly PCR Applications.
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    Chapter 2 Synthetic Gene Design Using Codon Optimization On-Line (COOL).
  4. Altmetric Badge
    Chapter 3 Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering.
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    Chapter 4 Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli.
  6. Altmetric Badge
    Chapter 5 Synthetic DNA
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    Chapter 6 BASIC: A Simple and Accurate Modular DNA Assembly Method.
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    Chapter 7 Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides.
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    Chapter 8 Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).
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    Chapter 9 PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly.
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    Chapter 10 The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis.
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    Chapter 11 Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria.
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    Chapter 12 Generation of DNA Constructs Using the Golden GATEway Cloning Method.
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    Chapter 13 Synthetic DNA
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    Chapter 14 Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR).
  16. Altmetric Badge
    Chapter 15 Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactions.
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    Chapter 16 Synthetic DNA
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    Chapter 17 Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA.
  19. Altmetric Badge
    Chapter 18 Selection of Error-Less Synthetic Genes in Yeast.
Attention for Chapter 7: Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides.
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Chapter title
Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides.
Chapter number 7
Book title
Synthetic DNA
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6343-0_7
Pubmed ID
Book ISBNs
978-1-4939-6341-6, 978-1-4939-6343-0
Authors

Cosimo Ducani, Björn Högberg

Editors

Randall A. Hughes

Abstract

Single-stranded oligonucleotides, or oligodeoxyribonucleotides (ODNs), are very important in several fields of science such as molecular biology, diagnostics, nanotechnology, and gene therapy. They are usually chemically synthesized. Here we describe an enzymatic method which enables us to synthesize pure oligonucleotides which can be up to several hundred long bases.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 9%
China 1 9%
Unknown 9 82%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 36%
Other 2 18%
Student > Doctoral Student 1 9%
Librarian 1 9%
Student > Master 1 9%
Other 1 9%
Unknown 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 45%
Biochemistry, Genetics and Molecular Biology 4 36%
Social Sciences 1 9%
Unknown 1 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 January 2018.
All research outputs
#19,594,120
of 24,093,053 outputs
Outputs from Methods in molecular biology
#8,407
of 13,601 outputs
Outputs of similar age
#319,370
of 428,053 outputs
Outputs of similar age from Methods in molecular biology
#694
of 1,072 outputs
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So far Altmetric has tracked 13,601 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,072 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.