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Mendeley readers
Chapter title |
Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR).
|
---|---|
Chapter number | 14 |
Book title |
Synthetic DNA
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6343-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6341-6, 978-1-4939-6343-0
|
Authors |
Sunil Chandran, Elaine Shapland |
Editors |
Randall A. Hughes |
Abstract |
The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR). The YHR method utilizes overlapping DNA parts that are assembled together by Saccharomyces cerevisiae via homologous recombination between designed overlapping regions. Using this method, we have successfully assembled up to 12 DNA parts in a single reaction. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
China | 1 | 5% |
Unknown | 18 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 21% |
Researcher | 4 | 21% |
Student > Ph. D. Student | 3 | 16% |
Student > Bachelor | 2 | 11% |
Other | 1 | 5% |
Other | 1 | 5% |
Unknown | 4 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 42% |
Biochemistry, Genetics and Molecular Biology | 7 | 37% |
Unknown | 4 | 21% |