Chapter title |
Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9.
|
---|---|
Chapter number | 2 |
Book title |
In Vitro Mutagenesis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6472-7_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6470-3, 978-1-4939-6472-7
|
Authors |
Kit-San Yuen, Chi-Ping Chan, Kin-Hang Kok, Dong-Yan Jin, Yuen KS, Chan CP, Kok KH, Jin DY, Yuen, Kit-San, Chan, Chi-Ping, Kok, Kin-Hang, Jin, Dong-Yan |
Editors |
Andrew Reeves |
Abstract |
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 nuclease (Cas9) system is a powerful genome-editing tool for both chromosomal and extrachromosomal DNA. DNA viruses such as Epstein-Barr virus (EBV), which undergoes episomal replication in human cells, can be effectively edited by CRISPR/Cas9. We have demonstrated targeted editing of the EBV genome by CRISPR/Cas9 in several lines of EBV-infected cells. CRISPR/Cas9-based mutagenesis and genome engineering of EBV provides a new method for genetic analysis, which has some advantages over bacterial artificial chromosome-based recombineering. This approach might also prove useful in the cure of EBV infection. In this chapter, we use the knockout of the BART promoter as an example to detail the experimental procedures for construction of recombinant EBV in human cells. |
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Unknown | 1 | 50% |
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Members of the public | 2 | 100% |
Mendeley readers
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Demographic breakdown
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Student > Master | 4 | 15% |
Researcher | 3 | 12% |
Other | 2 | 8% |
Student > Bachelor | 2 | 8% |
Other | 1 | 4% |
Unknown | 8 | 31% |
Readers by discipline | Count | As % |
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Mathematics | 1 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 1 | 4% |
Unknown | 6 | 23% |