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Mendeley readers
Chapter title |
Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method.
|
---|---|
Chapter number | 25 |
Book title |
In Vitro Mutagenesis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6472-7_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6470-3, 978-1-4939-6472-7
|
Authors |
Yongzhen Xia, Luying Xun |
Editors |
Andrew Reeves |
Abstract |
Site-directed mutagenesis has been widely used for the substitution, addition or deletion of nucleotide residues in a defined DNA sequence. QuikChange™ site-directed mutagenesis and its related protocols have been widely used for this purpose because of convenience and efficiency. We have recently demonstrated that the mechanism of the QuikChange™ site-directed mutagenesis process is different from that being proposed. The new mechanism promotes the use of partially overlapping primers and commercial PCR enzymes for efficient PCR and mutagenesis. |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Croatia | 1 | 3% |
United States | 1 | 3% |
Unknown | 32 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 7 | 21% |
Student > Ph. D. Student | 6 | 18% |
Researcher | 4 | 12% |
Student > Postgraduate | 2 | 6% |
Student > Master | 2 | 6% |
Other | 3 | 9% |
Unknown | 10 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 12 | 35% |
Agricultural and Biological Sciences | 5 | 15% |
Chemistry | 3 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Immunology and Microbiology | 1 | 3% |
Other | 1 | 3% |
Unknown | 11 | 32% |