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Mendeley readers
Chapter title |
Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent.
|
---|---|
Chapter number | 33 |
Book title |
In Vitro Mutagenesis
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6472-7_33 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6470-3, 978-1-4939-6472-7
|
Authors |
Toshifumi Minamoto |
Editors |
Andrew Reeves |
Abstract |
Heavy water is a form of water that contains a heavier isotope of hydrogen ((2)H, also known as deuterium, D) or oxygen ((17)O or (18)O). When using heavy water as a solvent, error-prone polymerase chain reaction (epPCR) can induce random mutations independent of the polymerase used or the composition of the PCR reaction mixture. This relatively new method can easily be combined with the existing epPCR methods to increase the rate of mutations. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 20% |
Researcher | 1 | 10% |
Other | 1 | 10% |
Student > Master | 1 | 10% |
Unknown | 5 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Agricultural and Biological Sciences | 2 | 20% |
Physics and Astronomy | 1 | 10% |
Unknown | 5 | 50% |