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Type-1 Diabetes

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Cover of 'Type-1 Diabetes'

Table of Contents

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    Book Overview
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    Chapter 286 Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.
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    Chapter 287 Histology of Type 1 Diabetes Pancreas.
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    Chapter 288 Fluorescence In Situ Hybridization with Concomitant Immunofluorescence in Human Pancreas.
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    Chapter 289 Type 1 Diabetes: Current Perspectives.
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    Chapter 290 Laser Capture and Single Cell Genotyping from Frozen Tissue Sections.
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    Chapter 291 Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants.
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    Chapter 292 Islet Autoantibody Analysis: Radioimmunoassays.
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    Chapter 293 Tracking Immunological Responses of Islet Antigen-Specific T Cells in the Nonobese Diabetic (NOD) Mouse Model of Type 1 Diabetes.
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    Chapter 294 Adoptive Transfer of Autoimmune Diabetes Using Immunodeficient Nonobese Diabetic (NOD) Mice.
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    Chapter 295 Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.
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    Chapter 296 Islet Autoantibody Detection by Electrochemiluminescence (ECL) Assay.
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    Chapter 307 Type 1 Diabetes High-Risk HLA Class II Determination by Polymerase Chain Reaction Sequence-Specific Primers.
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    Chapter 330 Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function
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    Chapter 331 The Gut Microbiome in the NOD Mouse
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    Chapter 339 Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes.
Attention for Chapter 288: Fluorescence In Situ Hybridization with Concomitant Immunofluorescence in Human Pancreas.
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Chapter title
Fluorescence In Situ Hybridization with Concomitant Immunofluorescence in Human Pancreas.
Chapter number 288
Book title
Type-1 Diabetes
Published in
Methods in molecular biology, December 2015
DOI 10.1007/7651_2015_288
Pubmed ID
Book ISBNs
978-1-4939-3641-0, 978-1-4939-3643-4
Authors

Jody Ye, Kathleen M. Gillespie

Editors

Kathleen M. Gillespie

Abstract

The ability to identify the presence of non-host cells in human pancreas with concomitant characterization of cell phenotype is particularly important to facilitate studies of transplantation and microchimerism resulted from pregnancy. The steps involved in processing tissue for fluorescence in situ hybridization (FISH) can however remove epitopes that are crucial for immunofluorescence and antigen retrieval strategies for immunofluorescence can negatively influence FISH. We describe a robust method to analyze X/Y chromosome constitution and cell phenotype simultaneously on the same pancreatic tissue section.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 50%
Student > Bachelor 1 25%
Professor 1 25%
Readers by discipline Count As %
Neuroscience 2 50%
Biochemistry, Genetics and Molecular Biology 1 25%
Pharmacology, Toxicology and Pharmaceutical Science 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 October 2017.
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#20,344,065
of 22,890,496 outputs
Outputs from Methods in molecular biology
#9,921
of 13,134 outputs
Outputs of similar age
#326,738
of 389,316 outputs
Outputs of similar age from Methods in molecular biology
#1,052
of 1,468 outputs
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