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Type-1 Diabetes

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Cover of 'Type-1 Diabetes'

Table of Contents

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    Book Overview
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    Chapter 286 Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.
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    Chapter 287 Histology of Type 1 Diabetes Pancreas.
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    Chapter 288 Fluorescence In Situ Hybridization with Concomitant Immunofluorescence in Human Pancreas.
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    Chapter 289 Type 1 Diabetes: Current Perspectives.
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    Chapter 290 Laser Capture and Single Cell Genotyping from Frozen Tissue Sections.
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    Chapter 291 Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants.
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    Chapter 292 Islet Autoantibody Analysis: Radioimmunoassays.
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    Chapter 293 Tracking Immunological Responses of Islet Antigen-Specific T Cells in the Nonobese Diabetic (NOD) Mouse Model of Type 1 Diabetes.
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    Chapter 294 Adoptive Transfer of Autoimmune Diabetes Using Immunodeficient Nonobese Diabetic (NOD) Mice.
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    Chapter 295 Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.
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    Chapter 296 Islet Autoantibody Detection by Electrochemiluminescence (ECL) Assay.
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    Chapter 307 Type 1 Diabetes High-Risk HLA Class II Determination by Polymerase Chain Reaction Sequence-Specific Primers.
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    Chapter 330 Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function
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    Chapter 331 The Gut Microbiome in the NOD Mouse
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    Chapter 339 Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes.
Attention for Chapter 296: Islet Autoantibody Detection by Electrochemiluminescence (ECL) Assay.
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Chapter title
Islet Autoantibody Detection by Electrochemiluminescence (ECL) Assay.
Chapter number 296
Book title
Type-1 Diabetes
Published in
Methods in molecular biology, December 2015
DOI 10.1007/7651_2015_296
Pubmed ID
Book ISBNs
978-1-4939-3641-0, 978-1-4939-3643-4
Authors

Liping Yu

Editors

Kathleen M. Gillespie

Abstract

Two fundamental aspects for precisely predicting the risk of developing type 1 diabetes by islet autoantibodies are assay sensitivity and disease specificity. We have recently developed electrochemiluminescent (ECL) insulin autoantibody (IAA) and GAD65 autoantibody (GADA) assays. ECL assays are sensitive, able to identify the initiation of islet autoimmunity earlier in life among high-risk young children before clinical onset of diabetes and are more disease specific because they are able to discriminate high-affinity, high-risk diabetes specific islet autoantibodies from low-affinity, low-risk autoantibodies.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Other 2 14%
Student > Doctoral Student 2 14%
Student > Ph. D. Student 2 14%
Student > Postgraduate 2 14%
Researcher 2 14%
Other 1 7%
Unknown 3 21%
Readers by discipline Count As %
Medicine and Dentistry 4 29%
Biochemistry, Genetics and Molecular Biology 2 14%
Psychology 1 7%
Nursing and Health Professions 1 7%
Immunology and Microbiology 1 7%
Other 1 7%
Unknown 4 29%