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Mendeley readers
| Chapter title |
Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.
|
|---|---|
| Chapter number | 295 |
| Book title |
Type-1 Diabetes
|
| Published in |
Methods in molecular biology, December 2015
|
| DOI | 10.1007/7651_2015_295 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3641-0, 978-1-4939-3643-4
|
| Authors |
James A. Pearson, F. Susan Wong |
| Editors |
Kathleen M. Gillespie |
| Abstract |
MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 20% |
| Researcher | 1 | 20% |
| Student > Postgraduate | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Agricultural and Biological Sciences | 1 | 20% |
| Chemistry | 1 | 20% |
| Medicine and Dentistry | 1 | 20% |
| Unknown | 1 | 20% |