Chapter title |
OSPREY Predicts Resistance Mutations Using Positive and Negative Computational Protein Design.
|
---|---|
Chapter number | 15 |
Book title |
Computational Protein Design
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6637-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6635-6, 978-1-4939-6637-0
|
Authors |
Adegoke Ojewole, Anna Lowegard, Pablo Gainza, Stephanie M. Reeve, Ivelin Georgiev, Amy C. Anderson, Bruce R. Donald, Ojewole, Adegoke, Lowegard, Anna, Gainza, Pablo, Reeve, Stephanie M, Georgiev, Ivelin, Anderson, Amy C, Donald, Bruce R, Reeve, Stephanie M., Anderson, Amy C., Donald, Bruce R. |
Editors |
Ilan Samish |
Abstract |
Drug resistance in protein targets is an increasingly common phenomenon that reduces the efficacy of both existing and new antibiotics. However, knowledge of future resistance mutations during pre-clinical phases of drug development would enable the design of novel antibiotics that are robust against not only known resistant mutants, but also against those that have not yet been clinically observed. Computational structure-based protein design (CSPD) is a transformative field that enables the prediction of protein sequences with desired biochemical properties such as binding affinity and specificity to a target. The use of CSPD to predict previously unseen resistance mutations represents one of the frontiers of computational protein design. In a recent study (Reeve et al. Proc Natl Acad Sci U S A 112(3):749-754, 2015), we used our OSPREY (Open Source Protein REdesign for You) suite of CSPD algorithms to prospectively predict resistance mutations that arise in the active site of the dihydrofolate reductase enzyme from methicillin-resistant Staphylococcus aureus (SaDHFR) in response to selective pressure from an experimental competitive inhibitor. We demonstrated that our top predicted candidates are indeed viable resistant mutants. Since that study, we have significantly enhanced the capabilities of OSPREY with not only improved modeling of backbone flexibility, but also efficient multi-state design, fast sparse approximations, partitioned continuous rotamers for more accurate energy bounds, and a computationally efficient representation of molecular-mechanics and quantum-mechanical energy functions. Here, using SaDHFR as an example, we present a protocol for resistance prediction using the latest version of OSPREY. Specifically, we show how to use a combination of positive and negative design to predict active site escape mutations that maintain the enzyme's catalytic function but selectively ablate binding of an inhibitor. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 25% |
Student > Ph. D. Student | 3 | 15% |
Student > Doctoral Student | 2 | 10% |
Student > Bachelor | 2 | 10% |
Student > Master | 2 | 10% |
Other | 3 | 15% |
Unknown | 3 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Agricultural and Biological Sciences | 3 | 15% |
Medicine and Dentistry | 2 | 10% |
Computer Science | 2 | 10% |
Chemistry | 2 | 10% |
Other | 2 | 10% |
Unknown | 4 | 20% |