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| Chapter title |
Production of Computationally Designed Small Soluble- and Membrane-Proteins: Cloning, Expression, and Purification.
|
|---|---|
| Chapter number | 3 |
| Book title |
Computational Protein Design
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6637-0_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6635-6, 978-1-4939-6637-0
|
| Authors |
Barsa Tripathy, Rudresh Acharya |
| Editors |
Ilan Samish |
| Abstract |
This book chapter focuses on expression and purification of computationally designed small soluble proteins and membrane proteins that are ordinarily difficult to express in good amounts for experiments. Over-expression of such proteins can be achieved by using the solubility tag such as maltose binding protein (MBP), Thioredoxin (Trx), and Gultathione-S-transferase (GST) fused to the protein of interest. Here, we describe and provide the protocols for cloning, expression and purification of such proteins using the solubility tag. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 1 | 20% |
| Researcher | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 2 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 40% |
| Agricultural and Biological Sciences | 1 | 20% |
| Unknown | 2 | 40% |