Chapter title |
Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer.
|
---|---|
Chapter number | 13 |
Book title |
The Tumor Microenvironment
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3801-8_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3799-8, 978-1-4939-3801-8
|
Authors |
Jitka Y. Sagiv, Sandra Voels, Zvi Granot |
Editors |
Josie Ursini-Siegel, Nicole Beauchemin |
Abstract |
Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 2% |
Unknown | 45 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 22% |
Student > Bachelor | 8 | 17% |
Student > Master | 6 | 13% |
Researcher | 3 | 7% |
Student > Doctoral Student | 2 | 4% |
Other | 1 | 2% |
Unknown | 16 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 9 | 20% |
Medicine and Dentistry | 6 | 13% |
Biochemistry, Genetics and Molecular Biology | 5 | 11% |
Agricultural and Biological Sciences | 4 | 9% |
Engineering | 2 | 4% |
Other | 3 | 7% |
Unknown | 17 | 37% |