Chapter title |
A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment.
|
---|---|
Chapter number | 5 |
Book title |
The Tumor Microenvironment
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3801-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3799-8, 978-1-4939-3801-8
|
Authors |
José A. Jiménez-Torres, David J. Beebe, Kyung E. Sung |
Editors |
Josie Ursini-Siegel, Nicole Beauchemin |
Abstract |
Microscale 3D in vitro systems have attracted significant interest as tools for cancer research because the microscale systems offer better organization of the cellular microenvironment and enhance throughput of the systems by lowering costs and reducing the amount of reagents and cells. Lumens (i.e., tubular structures) are ubiquitous in vivo being present in blood vessels, mammary ducts, prostate ducts, and the lymphatic system. Lumen structures of varying size and geometry are involved in key normal and disease processes including morphogenesis, angiogenesis, cancer development, and drug delivery. Therefore, there is a need for practical methods that create various lumen structures having different size and geometries to investigate how cells in the lumen structure respond to certain microenvironmental conditions during cancer development and progression. Here, we present a method to create multiple three-dimensional (3D) luminal structures, where parameters, such as size, geometry, and distance, can easily be controlled using simple poly-dimethylsiloxane (PDMS) micro-molds. |
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