Chapter title |
The Tumor Microenvironment
|
---|---|
Chapter number | 18 |
Book title |
The Tumor Microenvironment
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3801-8_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3799-8, 978-1-4939-3801-8
|
Authors |
Arendt, Lisa M, Lisa M. Arendt |
Editors |
Josie Ursini-Siegel, Nicole Beauchemin |
Abstract |
The tumor microenvironment plays a critical role in breast cancer growth and progression to metastasis. Here, we describe a method to examine stromal-epithelial interactions during tumor formation and progression utilizing human-derived mammary epithelial cells and breast stromal cells. This method outlines the isolation of each cell type from reduction mammoplasty tissue, the culture and genetic modification of both epithelial and stromal cells using lentiviral technology, and the method of humanizing and implantation of transformed epithelial cells into the cleared mammary fat pads of immunocompromised mice. This model system may be a useful tool to dissect signaling interactions that contribute to invasive tumor behavior and therapeutic resistance. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 23% |
Student > Doctoral Student | 2 | 15% |
Student > Bachelor | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Unknown | 6 | 46% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 4 | 31% |
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Nursing and Health Professions | 1 | 8% |
Unknown | 6 | 46% |